plvx ires puro vector  (Addgene inc)

Journal: bioRxiv

Article Title: Physiological function of Flo11p domains and the particular role of amyloid core sequences of this adhesin in Saccharomyces cerevisiae

doi: 10.1101/2021.04.01.438097

Figure Lengend Snippet: In panel (A) are shown AFM height image (a) and adhesion image (b) of a single cell from L69 strain expressing Flo11p defective of the N-terminus ( flo11-ΔNter ), C-terminus ( flo11-ΔCter ) or removed from the RR1 ( flo11-ΔRR1 ) or RR2 ( flo11-ΔRR2 ) domain as depicted in . In (B) is shown AFM height and adhesion images of a single cell from BY flo11Δ strain transformed with pYES2.1 carrying the chimeric FLO11 BY [RR2] L69 gene that corresponded to wild type FLO11 of BY4741 in which RR2 sequence from FLO11 of strain L69 has been inserted.

Article Snippet: The FLO11 ORF from BY4741 ( FLO11 BY ) and L69 ( FLO11 L69 ) were amplified by PCR using the primers FLO11_TOPO_f and FLO11_TOPO_r ( ) and ligated into pYES2.1 TOPO TA vector (ThermoFisher Scientific) between GAL1 promoter and CYC1 terminator according to the manufacture’s protocol.

Techniques: Expressing, Transformation Assay, Sequencing

Journal: bioRxiv

Article Title: Physiological function of Flo11p domains and the particular role of amyloid core sequences of this adhesin in Saccharomyces cerevisiae

doi: 10.1101/2021.04.01.438097

Figure Lengend Snippet: Yeast cells were initially cultivated in YNGal complemented with amino acids and uracil, except for BY flo11Δ and Σ flo11Δ expressing FLO11 BY or FLO11 L69 on a pYES2.1 plasmid for which uracil was omitted. Cells were harvested at the entrance in stationary phase and deposited as patches on agar plates which were made with (A) rich galactose medium (YP Gal) or (B) with synthetic glucose medium complemented with amino acids and uracil, except for BY flo11Δ and Σ flo11Δ expressing FLO11 BY on FLO11 L69 on a pYES2.1 plasmid for which uracil was omitted. Plates were incubated at 30°C for 8 days. They were then photographed before and after washing under a stream of water.

Article Snippet: The FLO11 ORF from BY4741 ( FLO11 BY ) and L69 ( FLO11 L69 ) were amplified by PCR using the primers FLO11_TOPO_f and FLO11_TOPO_r ( ) and ligated into pYES2.1 TOPO TA vector (ThermoFisher Scientific) between GAL1 promoter and CYC1 terminator according to the manufacture’s protocol.

Techniques: Expressing, Plasmid Preparation, Incubation

Journal: The Cell Surface

Article Title: Differences in fungal immune recognition by monocytes and macrophages: N -mannan can be a shield or activator of immune recognition

doi: 10.1016/j.tcsw.2020.100042

Figure Lengend Snippet: Strains used in this study.

Article Snippet: The S. cerevisiae optimized, galactose-inducible protein expression vector pYES2.1/V5-His-TOPO (Invitrogen, Paisley, UK) was used to express S. cerevisiae OCH1 in the S. cerevisiae och 1Δ null mutant strain.

Techniques: Transformation Assay